FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17374488
Sequences flagged as poor quality0
Sequence length35-155
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG5613500.09.024533145-149
CGGGGGG854000.06.684231145-149
GCGGGGG864450.06.5665245145-149
TGGGGGG700650.06.2206674145-149
GGGGCGG792400.06.0212436145-149
GGCGGGG895750.05.9586725145-149
GGGCGGG823500.05.9197483145-149
AGGGGGG400700.05.727221145-149
GGGGGCG847700.05.7201757145-149
GGGGGGT471700.05.6767282145-149
ATTTACG5500.00300199635.579496145-149
GGGTGGG832900.05.2089953145-149
GGTCGGG152400.05.063071145-149
CGATCGG30400.05.0501595145-149
GATCGGG78750.05.038375145-149
Produced by FastQC (version 0.11.5)