FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17374488
Sequences flagged as poor quality0
Sequence length35-155
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGG20050.06.238019145-149
CCGATCG11057.894414E-105.4768186145-149
CGGGGGG332100.05.272545145-149
GCGGGGG347500.05.213038145-149
TAGAGCG38450.05.1940756145-149
AGGGGGG254450.05.192879145-149
ACGCTTC61000.05.17130572
GATCGGT19950.05.1569943145-149
AAGCGCG100000.05.1239295145-149
Produced by FastQC (version 0.11.5)