FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15664274
Sequences flagged as poor quality0
Sequence length35-155
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG3993950.011.421284145-149
CGGGGGG615000.09.254698145-149
GCGGGGG639950.08.756448145-149
GATCGGG70050.08.035428145-149
CTGAACG46800.08.0003605145-149
GGGGCGG563250.07.9769244145-149
GGGCGGG608500.07.8049536145-149
TGGGGGG493750.07.781611145-149
GGCGGGG665500.07.725502145-149
GGGGGCG610100.07.3849645145-149
GGGGGGT337200.07.1466117145-149
AGGGGGG289600.07.1411867145-149
TCGGGGG138900.07.0917425145-149
CGTCTGA165950.06.472904145-149
CGATCGG35050.06.237354145-149
ACACGTC176500.06.184568140-144
GGTCGGG126250.06.1702027145-149
GGGCGCG199250.06.167081145-149
CCGATCG16300.06.166539145-149
TAGCTCG25750.06.14798145-149
Produced by FastQC (version 0.11.5)