FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16539103
Sequences flagged as poor quality0
Sequence length35-155
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG4893150.09.032575145-149
CGGGGGG752050.06.981875145-149
GCGGGGG771450.06.7698035145-149
GGGGCGG701700.06.3364964145-149
TGGGGGG608000.06.168608145-149
GGGCGGG743350.06.1221385145-149
GGCGGGG811600.05.9814587145-149
GGGGGCG748250.05.885852145-149
AGGGGGG359750.05.6011705145-149
GGGGGGT410150.05.579708145-149
GACGCTT69850.05.41141131
TATATCG13801.2732926E-115.246085145-149
ACGCTTA35301.1116572E-75.155212
GGTCGGG132250.05.1396427145-149
CGTTCGA21806.0280226E-45.13692676
CGCGGGG292900.05.01205145-149
GGGTGGG732100.05.007583145-149
Produced by FastQC (version 0.11.5)