FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13196396
Sequences flagged as poor quality0
Sequence length35-80
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCTTCATTGATTCCATCCGATTTCATTGGATGATGACTCCATTCGAG148090.1122200334091217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG2351.3706322E-615.93877971
CGTACGC2605.227743E-513.21453972
CCGAACG2150.00302014312.11765370
TTGCGTA2650.001206165211.13783911
CGAACTA2300.005694998411.1309363
GCGCTAT4406.9759153E-710.93770165
TATCGGG3801.8886449E-510.6800348
TATGCGG3800.00825266210.58886874
TGTATCG2450.00853357710.54125110
CCGCGTA2500.00989983710.33204560
TACTACG2850.00236580410.27270625
TAACGGG2900.002749742210.08820614
GTCGCGA3357.9122366E-49.86215445
GTACGAT3100.0044356229.5215297
ACGGCGC5853.1520358E-79.38806429
TAACGTA3550.00126274389.37123963
TGCGCTA4451.0393243E-49.14348364
CGATATA3750.00211654238.854650510
TTGAGTC113750.08.74238274
TAAACCG7954.95435E-58.67659474
Produced by FastQC (version 0.11.5)