FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13196396
Sequences flagged as poor quality0
Sequence length35-80
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG2951.2229975E-613.64821711
TACGCGG3406.0029197E-611.84161216
CGCACGT4551.6264924E-411.70313174
TCGCTAC4802.1444248E-710.6754336
GTTACGC3154.4935106E-410.48761143
TATCACG4409.466165E-69.9820712
TACCGGT3000.0036146659.7604325
GTAACCG6401.1793418E-79.15290322
TCTATCG5206.8358386E-69.1505099
GGTTCGG9906.146129E-78.964519574
TATGCGG5450.0094414828.68489274
TAACCGC7258.672214E-88.582587
CGACCTT6907.678084E-48.57475874
CGCCATT24150.08.57475874
ATAACCG6903.7424434E-78.48736
ATGCGCG3900.00324555658.44662413
GGTAACG4802.560257E-48.41449645
TACCGTC6450.00398936458.25569774
CGATATT6257.764E-68.19941618
AGTCGCT9503.1946438E-58.09637674
Produced by FastQC (version 0.11.5)