FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17249749
Sequences flagged as poor quality0
Sequence length35-80
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAT21550.08.16114274
ATTGCCG17405.284164E-97.173151574
AACGGTC9050.00214397846.89573674
AAACGCG10159.682868E-46.70736274
GCGTATG11656.610007E-46.330733374
CGATCGT7709.052187E-46.1680975
GGCGAAT11300.00324370976.02475474
TGCGAAA26600.06.00941270
GTGCGAA32450.05.84252670
CCTTTCG42750.05.83919774
AGTCACG57850.05.7860974
CGAAATC23907.65649E-85.69704874
GGACTCG101600.05.58396774
AAATTCG9600.00121218925.570134672
TCGAATT17609.7135766E-55.479902374
GCGAAAA17201.0076292E-75.38839267
CGAAAAC14752.5102938E-55.330568373
AGCCGAA12657.0347836E-55.281410769
AGGCGAT14200.006973465.19387674
TAACGGT12352.5067327E-45.144240471
Produced by FastQC (version 0.11.5)