FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20664827
Sequences flagged as poor quality0
Sequence length35-80
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT12950.011.5263674
TATGCCG18050.011.45022274
TCGTATG22750.09.58938674
CGTATGC25650.09.1766774
CGATAAT39100.08.2142421
CGTAACG5953.4456833E-58.04772751
ACCGCGC27650.07.5445631
CGTATGG11659.649973E-67.391863374
GGCTAAT64450.07.24850851
CGACGTT13407.8216544E-117.08123717
GCTAATT109700.06.98673441
AGTCGTC28700.06.87006121
CGTCGTG42600.06.861240424
CCGTCTT49200.06.82400132
GCGTGAG98000.06.81940222
CCGTTTT36200.06.7110039
ACAGGCG112200.06.53858418
ACGTAAT13555.2532414E-96.46386863
GGATTAC159500.06.438613413
GGGATTA167300.06.38038212
Produced by FastQC (version 0.11.5)