FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20664827
Sequences flagged as poor quality0
Sequence length35-80
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGG12804.7293724E-109.21649374
CGCACGT7752.7036387E-48.37214274
AGTCGTC33650.08.04133321
GGCTAAT64550.07.30456071
CGATAAT36750.07.0615761
CCGTCTT52550.06.88590432
TATCGGT11252.690238E-46.81610874
TAGCGCT11405.4929842E-86.73316535
CCCGTCT56550.06.5927741
ATAGGGG67200.06.583208674
CCCTCTT489900.06.58051162
AAGGACC110400.06.555315514
CCCCTCT496050.06.5358011
GACTTAG49850.06.4524165
TCACCGA308850.06.30786413
CGACCCA35050.06.29805748
CGGTATA21500.06.29457923
TCCTGTC625400.06.25942338
GTTTCGT10450.00642587156.20900574
GTTATCG13659.480209E-76.103447473
Produced by FastQC (version 0.11.5)