FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20433519
Sequences flagged as poor quality0
Sequence length20-80
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGC4000.00313157988.48012762
GTACGAA4450.0094876377.495672754
CGCATAT14705.969014E-86.703776473
CGTATGC14900.0093021675.478356474
GGCGCAT27601.2853488E-65.42211774
AAACCCC81150.05.36471674
ATTGCCG38051.3515091E-95.363174474
TCATACG17150.00759153955.156256774
Produced by FastQC (version 0.11.5)