FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8076060
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAATT37050.08.3914861
TATCGAG5501.1497914E-48.07309264
CGCATAT4850.00243324747.62954271
CTAATTT39950.07.5937082
CGATAAT25950.07.55629359
ATTACGT5750.00162677857.078318651
TTCGATA30650.07.0009527
TCGATAA35450.06.57481968
TTCGCCT7455.2146113E-46.4557497
TTAGGCT7556.130155E-46.37020253
GTAGAGA73900.06.3106471
GGATATC7100.00212827586.2556251
TAATTTT61550.06.25118163
AATCGCA59850.06.2460431
GGCTAAT22950.06.12842271
GCACACG134850.06.11934474
ACACGTC108750.06.090805574
CGTCTGA82900.06.025940473
CGCATAG22200.06.000345761
GTCTGAA91250.05.96122474
Produced by FastQC (version 0.11.5)