FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32279264
Sequences flagged as poor quality0
Sequence length35-80
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTTCG139500.08.64430674
AAGACGG126450.07.29782774
CGATCGT16200.06.99857918
CGCATAG36850.06.109854771
CTAACGC14853.3681185E-45.71859874
GACGCTT96250.05.70004031
CAAGACG125950.05.66365974
ACGACGG20053.7705067E-65.647293674
TCGCTCG9500.00183732925.3897553
ACGCAAT14051.260731E-55.271555463
CGACTCG23657.094059E-115.257395313
ACAAGAC177700.05.161219674
CAAGTCG29101.6919694E-75.058305774
GGACTCG316900.05.02005974
Produced by FastQC (version 0.11.5)