FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16760071
Sequences flagged as poor quality0
Sequence length35-155
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCG47500.07.4421577145-149
CGATCGG24900.07.428603145-149
AGCGCGG92850.07.326729145-149
AAGCGCG105650.07.3144617145-149
CGAGCGG44550.06.458689145-149
GCGGGGG346900.06.268237145-149
CGGGGGG330750.06.2573957145-149
AGAGCGG274750.06.193793145-149
GCGCGTC80550.06.072611145-149
TAGCTCG35150.05.905546145-149
ATCGGAC27200.05.893723145-149
GATCGTA34700.05.863673145-149
CCGATCG11252.1827873E-115.846036145-149
GGGGGGG2547050.05.771847145-149
GGGTCGG91650.05.7632127145-149
TGGGGGG353900.05.7609634145-149
AGGGGGG257950.05.7287173145-149
GCGAGCG36950.05.673483145-149
GGGGCGG334900.05.652083145-149
GGTCGGG93700.05.637123145-149
Produced by FastQC (version 0.11.5)