FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13607500
Sequences flagged as poor quality0
Sequence length35-155
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG3929600.09.511876145-149
CGGGGGG574050.07.6698575145-149
GCGGGGG595600.07.089436145-149
GACGCTT64950.06.64243561
GGGGCGG516450.06.550964145-149
TATATCG12300.06.4274197145-149
TGGGGGG477350.06.391116145-149
GGGCGGG553050.06.3666735145-149
AGGGGGG287900.06.336917145-149
GGGGGCG553150.06.1822886145-149
CCGATCG11401.8189894E-126.045764145-149
GATCGGG55150.05.9545183145-149
GGGGGGT330300.05.885552145-149
CGATCGG21700.05.791741145-149
GGCGGGG696200.05.770943145-149
ACGCTTC55600.05.59686662
GATCGGA142600.05.5776834135-139
TCGGGGG124950.05.5483904145-149
GGGCGCG164550.05.4327264145-149
GGGTGGG581200.05.3991146145-149
Produced by FastQC (version 0.11.5)