FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13607500
Sequences flagged as poor quality0
Sequence length35-155
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGG14250.09.32399145-149
CCGATCG7253.45608E-117.330585145-149
CGGGGGG209250.06.7794943145-149
GACGCTT76100.06.7024031
GATCGTA30400.06.3878293145-149
CCGGATT14506.3566654E-46.3512341
AAGCGCG59950.06.239722145-149
AGATCGT64850.06.1465006145-149
GCGGGGG229000.06.096611145-149
GGGGCGG222500.05.842926145-149
GGGGGGG1747850.05.7866716145-149
CGTCGGG67000.05.766208145-149
AGGGGGG173600.05.6872287145-149
GGGCGGG228100.05.6815557145-149
GGGGGGT163800.05.6655917145-149
TGGGGGG243150.05.632534145-149
GCGCGTC48550.05.5997124145-149
ACGCTTC71000.05.58642772
ATGCGCG11351.9281288E-105.5830193145-149
CGGTCGG17050.05.514889145-149
Produced by FastQC (version 0.11.5)