FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19904648
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG3654.0599843E-914.19142674
TCGTATG22150.09.855556573
TATGCCG18650.08.53085373
TATCGTA8601.567605E-88.1744473
CTCGTAT14150.07.844661772
GCGCGTA5205.5776467E-47.827061772
CGTATGC31350.07.43522174
CCTAACG6850.00142591836.48209567
CGGATAA7455.211323E-46.456214474
GTCGTAT14159.886207E-96.27572972
CCGTCTA51850.05.99406474
CTTGCGA8150.00153929265.90136714
CGTATAA15103.9262886E-85.880898572
CCGTATA11351.3105791E-55.868048771
ATCGTAT15952.0425432E-85.799227774
CGTCTAA47550.05.602820467
CAATACG10408.5480657E-45.336552673
GACGTAT10750.00132929525.16280473
AATATCG18002.78711E-75.138902773
CGCATAG16155.1463267E-65.04042671
Produced by FastQC (version 0.11.5)