FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30528622
Sequences flagged as poor quality0
Sequence length80
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG34350.08.29387574
CTCGTAT18400.07.84224474
TATGCCG25600.07.081897774
CGTATGC42400.06.71921374
CGATCCG5600.0098526356.60731164
CGCAAAT56750.06.58489874
ACGATAG7354.4196466E-46.54439459
TACGCAA98350.06.24522270
TAACGTA24100.06.14126571
GTACGCA104350.05.815192769
ACGCAAA111600.05.76870874
CGTACGA15658.332063E-85.67428470
ATCGTAT27650.05.620229773
GTACGAA21802.910383E-115.6010669
CGTACGC97200.05.44356368
TATCGTA13708.245941E-65.40135874
ACGTACG112550.05.161494367
ACGTACC23903.7471182E-105.108690774
AATCGCG13201.442623E-45.04559168
Produced by FastQC (version 0.11.5)