FastQCFastQC Report
Fri 10 May 2024
EGAF00005490691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524055428
Total Bases53.4 Gbp
Sequences flagged as poor quality0
Sequence length102
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA7925310.1512303771043089Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAA6799930.1297559310844501Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6034390.11514793431354364No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)