FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56143
Sequences flagged as poor quality0
Sequence length301
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT1060.18880359083055767No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT910.16208610156208253No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT900.16030493561085085No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT880.1567426037083875No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT880.1567426037083875No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT860.15318027180592414No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT830.14783677395222913No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT830.14783677395222913No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT820.14605560800099746No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT810.14427444204976578No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT810.14427444204976578No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT810.14427444204976578No Hit
TTCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT800.1424932760985341No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT790.14071211014730242No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT770.13714977824483907No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT770.13714977824483907No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT770.13714977824483907No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT760.1353686122936074No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT750.1335874463423757No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT750.1335874463423757No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT750.1335874463423757No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT750.1335874463423757No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT750.1335874463423757No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT740.13180628039114406No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT740.13180628039114406No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT740.13180628039114406No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT730.13002511443991235No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT730.13002511443991235No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT730.13002511443991235No Hit
CCTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT730.13002511443991235No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT720.1282439484886807No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT720.1282439484886807No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT720.1282439484886807No Hit
ATAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT720.1282439484886807No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT720.1282439484886807No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT710.126462782537449No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT710.126462782537449No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT710.126462782537449No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT710.126462782537449No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT710.126462782537449No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.12468161658621733No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.12468161658621733No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.12468161658621733No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.12468161658621733No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT700.12468161658621733No Hit
AGGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.12290045063498567No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.12290045063498567No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.12290045063498567No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.12290045063498567No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT690.12290045063498567No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT680.12111928468375398No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT680.12111928468375398No Hit
ACGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT680.12111928468375398No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT680.12111928468375398No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT680.12111928468375398No Hit
TCCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT680.12111928468375398No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT680.12111928468375398No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT680.12111928468375398No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT680.12111928468375398No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT670.11933811873252231No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT670.11933811873252231No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT670.11933811873252231No Hit
AACCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT670.11933811873252231No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT670.11933811873252231No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT670.11933811873252231No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT660.11755695278129062No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT660.11755695278129062No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT660.11755695278129062No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT660.11755695278129062No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT660.11755695278129062No Hit
GTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT660.11755695278129062No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT650.11577578683005896No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT650.11577578683005896No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT650.11577578683005896No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT650.11577578683005896No Hit
GGTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT650.11577578683005896No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT650.11577578683005896No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT650.11577578683005896No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT650.11577578683005896No Hit
AGTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT640.1139946208788273No Hit
ATTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT640.1139946208788273No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT640.1139946208788273No Hit
TCTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT630.1122134549275956No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT630.1122134549275956No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT630.1122134549275956No Hit
GTGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT630.1122134549275956No Hit
AGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT630.1122134549275956No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT620.11043228897636394No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT620.11043228897636394No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT620.11043228897636394No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT620.11043228897636394No Hit
TCGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT610.10865112302513225No Hit
ATCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT610.10865112302513225No Hit
GGGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT610.10865112302513225No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT610.10865112302513225No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT610.10865112302513225No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT610.10865112302513225No Hit
AGTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT610.10865112302513225No Hit
TGTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT610.10865112302513225No Hit
AAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT610.10865112302513225No Hit
CGTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT600.10686995707390057No Hit
CTCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT600.10686995707390057No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT600.10686995707390057No Hit
AGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT600.10686995707390057No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT600.10686995707390057No Hit
AAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT600.10686995707390057No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT600.10686995707390057No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT600.10686995707390057No Hit
GGTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT590.1050887911226689No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT590.1050887911226689No Hit
TGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT590.1050887911226689No Hit
TTAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT590.1050887911226689No Hit
CATCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT590.1050887911226689No Hit
CACCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT590.1050887911226689No Hit
CCGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT590.1050887911226689No Hit
AAAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT590.1050887911226689No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT580.10330762517143721No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT580.10330762517143721No Hit
CGCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT580.10330762517143721No Hit
GAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT580.10330762517143721No Hit
GTCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT570.10152645922020555No Hit
CGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT570.10152645922020555No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT570.10152645922020555No Hit
GCGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT570.10152645922020555No Hit
ATCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT570.10152645922020555No Hit
TGGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGT570.10152645922020555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTA1250.0295.000032
GAGCCTA550.0295.000032
CGTCCTA1100.0295.000032
GTGCCTA550.0295.000032
TCGCCCT255.2386895E-10295.01
GCCCCCT206.132905E-8295.01
CGGGTGG108.443617E-4295.09
GTCGCCT255.2386895E-10295.01
CGAACCT450.0295.01
CAACGGG108.443617E-4295.06
TTTACCT303.6379788E-12295.01
TTAGCCT157.2037838E-6295.01
GATCCCT206.132905E-8295.01
CAACCTA1000.0295.02
GCGCCTA450.0295.02
GCGCCCT108.443617E-4295.01
TAGCCTA500.0295.02
CGTTCCT350.0295.01
GACCCCT255.2386895E-10295.01
ACGGGAT108.443617E-4295.07
Produced by FastQC (version 0.11.5)