FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64576
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC920.14246778989098116No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC860.13317641228939545No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC830.1285307234886026No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC780.12078790882061446No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.11923934588701686No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC770.11923934588701686No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.11769078295341923No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC760.11769078295341923No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC740.11459365708622399No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC730.11304509415262637No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC720.11149653121902874No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10994796828543113No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10994796828543113No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10994796828543113No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC710.10994796828543113No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC700.1083994053518335No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC690.10685084241823589No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC680.10530227948463826No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC680.10530227948463826No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10375371655104064No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC670.10375371655104064No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC660.102205153617443No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1006565906838454No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1006565906838454No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1006565906838454No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC650.1006565906838454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTA700.0295.000032
GTCACCT350.0295.000031
GCCCTAC2800.0295.000033
GGGCCTA700.0295.000032
TGATCCT400.0295.01
TCGCCTA900.0295.02
GCCCCTA1100.0295.02
ATCCTCG108.446562E-4295.03
AGTCCTA650.0295.02
GTCGCCT255.2568794E-10295.01
GTACCCT255.2568794E-10295.01
TGCCCCT157.2088114E-6295.01
AGATCCT108.446562E-4295.01
ACGCCTA1050.0295.02
AGCCCTA900.0295.02
TAGACCT157.2088114E-6295.01
ATTACCT108.446562E-4295.01
CTCCCTA800.0295.02
TTGTCCT450.0295.01
CACGCCT450.0295.01
Produced by FastQC (version 0.11.5)