FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75116
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT990.13179615527983388No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT980.13046488098407796No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT980.13046488098407796No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT970.12913360668832208No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT940.12513978380105437No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT920.12247723520954258No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT920.12247723520954258No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.11981468661803078No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.11981468661803078No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.11981468661803078No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT880.11715213802651898No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT870.11582086373076309No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC860.11448958943500719No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT860.11448958943500719No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT860.11448958943500719No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT850.11315831513925129No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT840.1118270408434954No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT840.1118270408434954No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT830.11049576654773949No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT830.11049576654773949No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT820.1091644922519836No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT820.1091644922519836No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT820.1091644922519836No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT810.10783321795622769No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC810.10783321795622769No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC810.10783321795622769No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT810.10783321795622769No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT790.10517066936471592No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC790.10517066936471592No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT790.10517066936471592No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC790.10517066936471592No Hit
GCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT790.10517066936471592No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT780.10383939506896No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT780.10383939506896No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT780.10383939506896No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT770.10250812077320412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCTA1250.0295.000032
GAACCTA950.0295.000032
CCCCCTA1250.0295.000032
TTGTCCT255.2568794E-10295.01
AATCCTA1350.0295.02
ACGCCTA1100.0295.02
TGCACCT450.0295.01
ATCTCCT450.0295.01
TGACCTA1350.0295.02
CTCCCTA650.0295.02
GTACCTA1400.0295.02
GGACCTA1000.0295.02
CGCCTAC4000.0295.03
ACGTCCT700.0295.01
GGCGCCT255.2568794E-10295.01
GGTCCCT500.0295.01
CATCCCT255.2568794E-10295.01
TAGCCCT450.0295.01
GAGCCTA900.0295.02
ACTCCTA900.0295.02
Produced by FastQC (version 0.11.5)