FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82468
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1420.17218800019401465No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1380.16733763399136634No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1350.16369985933938014No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1280.1552117184847456No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1280.1552117184847456No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1260.15278653538342146No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1250.15157394383275938No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1240.1503613522820973No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1220.14793616918077315No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1210.14672357763011107No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1210.14672357763011107No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1200.14551098607944898No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1190.14429839452878693No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1180.14308580297812484No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1180.14308580297812484No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1170.1418732114274628No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1170.1418732114274628No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1170.1418732114274628No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1160.1406606198768007No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1150.13944802832613862No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1140.13823543677547656No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1140.13823543677547656No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1140.13823543677547656No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1130.13702284522481448No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1130.13702284522481448No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1130.13702284522481448No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1120.1358102536741524No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1110.1345976621234903No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1110.1345976621234903No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1100.13338507057282825No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1090.13217247902216617No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1090.13217247902216617No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1090.13217247902216617No Hit
CGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1090.13217247902216617No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1080.1309598874715041No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1070.12974729592084203No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1070.12974729592084203No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1060.12853470437017994No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1060.12853470437017994No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1050.12732211281951789No Hit
TGTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1050.12732211281951789No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1050.12732211281951789No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1040.1261095212688558No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1040.1261095212688558No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1040.1261095212688558No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1040.1261095212688558No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1030.12489692971819372No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1020.12368433816753165No Hit
AGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1020.12368433816753165No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1020.12368433816753165No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1010.12247174661686958No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1010.12247174661686958No Hit
AAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1010.12247174661686958No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1010.12247174661686958No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1010.12247174661686958No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1010.12247174661686958No Hit
TCTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1010.12247174661686958No Hit
CCTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1000.1212591550662075No Hit
CTGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1000.1212591550662075No Hit
TATACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT1000.1212591550662075No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT990.12004656351554542No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT990.12004656351554542No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT980.11883397196488334No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT980.11883397196488334No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT980.11883397196488334No Hit
GAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT980.11883397196488334No Hit
CCGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT980.11883397196488334No Hit
GTTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT980.11883397196488334No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT970.11762138041422128No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT970.11762138041422128No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT970.11762138041422128No Hit
GTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT960.11640878886355921No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT960.11640878886355921No Hit
ACCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT960.11640878886355921No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT960.11640878886355921No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT960.11640878886355921No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT960.11640878886355921No Hit
TTGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT960.11640878886355921No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT950.11519619731289713No Hit
CCCTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT950.11519619731289713No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT950.11519619731289713No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT950.11519619731289713No Hit
CGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT950.11519619731289713No Hit
CCCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT940.11398360576223504No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT940.11398360576223504No Hit
TAAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT930.11277101421157297No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT930.11277101421157297No Hit
TAGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT930.11277101421157297No Hit
ACGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT930.11277101421157297No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT920.1115584226609109No Hit
AGCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT920.1115584226609109No Hit
CCAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT920.1115584226609109No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT920.1115584226609109No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT910.11034583111024883No Hit
TGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT910.11034583111024883No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT910.11034583111024883No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT910.11034583111024883No Hit
CATCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.10913323955958674No Hit
AAGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.10913323955958674No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.10913323955958674No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.10913323955958674No Hit
AAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.10913323955958674No Hit
GACACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.10913323955958674No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.10913323955958674No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.10913323955958674No Hit
TCAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.10913323955958674No Hit
AGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.10913323955958674No Hit
TTCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT900.10913323955958674No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT890.10792064800892466No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT890.10792064800892466No Hit
CGGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT890.10792064800892466No Hit
CAAGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT890.10792064800892466No Hit
TCGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT890.10792064800892466No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT890.10792064800892466No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC890.10792064800892466No Hit
TGGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT890.10792064800892466No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC890.10792064800892466No Hit
CTTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT880.1067080564582626No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT880.1067080564582626No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT880.1067080564582626No Hit
ATCGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT880.1067080564582626No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT880.1067080564582626No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT880.1067080564582626No Hit
GGTACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT880.1067080564582626No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC870.10549546490760053No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT870.10549546490760053No Hit
CGAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT860.10428287335693845No Hit
ATGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT860.10428287335693845No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC850.10307028180627636No Hit
AGACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT850.10307028180627636No Hit
CGTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT850.10307028180627636No Hit
ATCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT850.10307028180627636No Hit
GGACCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT850.10307028180627636No Hit
TAGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT850.10307028180627636No Hit
TTGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT850.10307028180627636No Hit
ATGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT840.10185769025561431No Hit
ATTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT840.10185769025561431No Hit
CGATCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT840.10185769025561431No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC840.10185769025561431No Hit
AAGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT840.10185769025561431No Hit
GGGGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT840.10185769025561431No Hit
GTGCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT830.10064509870495222No Hit
TGTCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT830.10064509870495222No Hit
CGTTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT830.10064509870495222No Hit
GAAACCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT830.10064509870495222No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT830.10064509870495222No Hit
TTTGCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT830.10064509870495222No Hit
CTCCCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAAAGT830.10064509870495222No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCTAGCC830.10064509870495222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCT550.0295.000031
CCCCTAC4100.0295.000033
CGGCCTA850.0295.000032
CGGCCCT550.0295.000031
GGCCCTA850.0295.000032
CCGCCTA1100.0295.000032
GTTGATT600.0295.0295
GCCCCTA900.0295.02
ATCCTAG108.4508077E-4295.03
TTCTACG108.4508077E-4295.03
AGTCCTA1000.0295.02
GTACCTA1500.0295.02
GATACCT206.150367E-8295.01
GTCGCCT350.0295.01
AGATCCT108.4508077E-4295.01
CGAACCT255.275069E-10295.01
GTCGATA108.4508077E-4295.0295
TCCGCCT500.0295.01
GTACAGT157.216062E-6295.0295
ATCTACG108.4508077E-4295.03
Produced by FastQC (version 0.11.5)