FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98133
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCCT550.0295.000031
TCGCCTA850.0295.02
CGAACCT157.2202347E-6295.01
CGTTCCT600.0295.01
GCACCCT255.275069E-10295.01
GAGCCTA1050.0295.02
TTGCCTA1000.0295.02
GGCGCCT255.275069E-10295.01
CGTCCTA1400.0295.02
TGATCCT108.453252E-4294.999971
GGATCCT206.1563696E-8294.999971
CCTACGA108.453252E-4294.999975
TACGCCT108.453252E-4294.999971
CGCTACG108.453252E-4294.999973
GTCCTAC7050.0292.90783
ACCTACG26350.0292.201144
CCTACGG92000.0290.831545
TCCTACG26650.0290.57224
GCCTACG17700.0290.000034
CCCTACG21550.0289.524384
Produced by FastQC (version 0.11.5)