FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98133
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA11381.159650678161271No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA11261.1474223757553523No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA10031.0220822760946877No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA9901.0088349484882762No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA9760.9945685956813712No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA9360.9538075876616429No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA9310.9487124616591769No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA8990.9161036552433941No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA8150.8305055384019646No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA7680.7826113539787839No Hit
GGACTACCCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG7370.7510215727634945No Hit
GGACTACCCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6710.6837659095309427No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA6190.6307765991052958No Hit
GGACTACTCGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG6100.621605372300857No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA6040.6154912210978978No Hit
GGACTACTCGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5900.6012248682909929No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA5890.6002058430904996No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA5850.5961297422885268No Hit
GGACTACCAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5730.5839014398826083No Hit
GGACTACCAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5630.5737111878776762No Hit
GGACTACTAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5520.562501910672251No Hit
GGACTACTAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5270.5370262806599208No Hit
GGACTACCGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5210.5309121294569614No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA5080.5176648018505497No Hit
GGACTACCGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG5040.5135887010485769No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA4860.49524624743969914No Hit
GGACTACCCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4410.44939011341750484No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT4250.4330857102096135No Hit
GGACTACTGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3890.396400802991858No Hit
GGACTACTGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3820.3892676265884055No Hit
GGACTACCCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA3710.3780583493829802No Hit
GGACTACCCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA3700.37703932418248703No Hit
GGACTACACGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3600.36684907217755497No Hit
GGACTACTCGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA3540.3607349209745957No Hit
GGACTACACGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3510.3576778453731161No Hit
GGACTACTCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3490.35563979497212966No Hit
GGACTACCAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA3480.3546207697716365No Hit
GGACTACTCGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3450.3515636941701568No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3350.34137344216522475No Hit
GGACTACTCGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA3300.3362783161627587No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3260.33220221536078587No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA3160.3220119633558538No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3150.32099293815536056No Hit
GGACTACCAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA3130.31895488775437414No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT3120.31793586255388095No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA3060.31182171135092174No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3040.3097836609499353No Hit
GGACTACAAGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG3030.3087646357494421No Hit
GGACTACTAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA3020.3077456105489489No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2970.30265048454648286No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2880.2934792577420439No Hit
GGACTACCGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2810.2863460813385915No Hit
GGACTACCGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2790.2843080309376051No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2770.28226998053661867No Hit
GGACTACTAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2730.2781938797346458No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2700.2751368041331662No Hit
GGACTACCGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2640.26902265293020694No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2480.25271824972231566No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2400.24456604811837No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2340.23845189691541072No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2330.23743287171491748No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2330.23743287171491748No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2320.2364138465144243No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2310.2353948213139311No Hit
GGACTACCGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2300.2343757961134379No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2250.22928067011097186No Hit
GGACTACTGGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2230.22724261970998544No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2220.2262235945094922No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2180.22214749370751938No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2180.22214749370751938No Hit
GGACTACACGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA2110.21501431730406692No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2110.21501431730406692No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC2080.2119572417025873No Hit
GGACTACTGGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2060.2099191913016009No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1960.1997289392966688No Hit
GGACTACACGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1930.1966718636951892No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1910.19463381329420276No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1880.19157673769272315No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1880.19157673769272315No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1870.19055771249222994No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1860.18953868729173673No Hit
GGACTACTGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1860.18953868729173673No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1860.18953868729173673No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1840.1875006368907503No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1820.18546258648976388No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1820.18546258648976388No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1810.18444356128927067No Hit
GGACTACAAGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1770.18036746048729785No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1760.17934843528680464No Hit
GGACTACCCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1750.17832941008631142No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1740.17731038488581824No Hit
GGACTACTGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1730.17629135968532503No Hit
GGACTACAAGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1720.17527233448483182No Hit
GGACTACAGGGGTTTCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1710.1742533092843386No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1690.17221525888335218No Hit
GGACTACCCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1690.17221525888335218No Hit
GGACTACTCGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1660.16915818328187257No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1650.16813915808137936No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1630.16610110768039293No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1580.1610059816779269No Hit
GGACTACAGGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1550.15794890607644727No Hit
GGACTACCAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1540.15692988087595408No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1530.15591085567546087No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1530.15591085567546087No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1500.15285378007398123No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1500.15285378007398123No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1480.1508157296729948No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1480.1508157296729948No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1470.1497967044725016No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1460.14877767927200838No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1460.14877767927200838No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1460.14877767927200838No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1430.14572060367052878No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1410.14368255326954238No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1410.14368255326954238No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1390.14164450286855595No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1390.14164450286855595No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1380.14062547766806274No Hit
GGACTACACGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1360.13858742726707632No Hit
GGACTACTCGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1350.1375684020665831No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1340.1365493768660899No Hit
GGACTACTAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1340.1365493768660899No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1340.1365493768660899No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1330.13553035166559668No Hit
GGACTACCAGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1330.13553035166559668No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1300.13247327606411707No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1300.13247327606411707No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1270.12941620046263744No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1260.12839717526214423No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1230.12534009966066462No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1220.1243210744601714No Hit
GGACTACCGGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1210.12330204925967819No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1200.122283024059185No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGAATCTTCGCTATTGTGTATTC1190.12126399885869178No Hit
GGACTACCCGGGTTTCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1190.12126399885869178No Hit
GGACTACCCGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1190.12126399885869178No Hit
GGACTACTAGGGTATCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1160.11820692325721215No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1140.11616887285622574No Hit
GGACTACCCGGGTATCTAATGGTCCCTGGAGTTTCTCGGCACAGAGCTCA1110.1131117972547461No Hit
GGACTACCGGGGTTTCTACATTGGTAAGACATAAGCCTGGGGTTCCTGGC1110.1131117972547461No Hit
GGACTACTCGGGTATCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1110.1131117972547461No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGTGCCTTAGCTGTTGTGTCCTC1070.10903569645277329No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1060.10801667125228008No Hit
GGACTACAGGGGTATCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA1050.10699764605178687No Hit
GGACTACCCGGGTATCTAATGGCTGACAGATTATCCTGTGTGCTTGGAGG1050.10699764605178687No Hit
GGACTACCCGGGTTTCTAACACCGGATGTGCTCTGACTCGCCCACTTCCG1030.10495959565080044No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC1020.10394057045030725No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGTTATTCTGTGTTC990.10088349484882761No Hit
GGACTACAGGGGTTTCTAATAGCTGAAGCTTAGCATGTCTCCTGATCTTA990.10088349484882761No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGGT4350.0295.07
AGTTATC255.275069E-10295.0295
GGCCTAC157.2202347E-6295.01
GGACTAC92650.0294.68161
CGGGTAT18800.0293.430859
CCGGGTT8500.0293.26478
ACACGGG7700.0293.084446
CACGGGT7650.0293.071877
GGGGTAT13800.0292.86239
GACTACC41800.0292.52992
ACCCGGG15950.0292.22576
CGGGTTT18450.0291.802159
CTCGGGT13650.0291.758247
CTACCGG11950.0291.29714
CCCGGGT15900.0291.28937
CTACCCG16150.0290.433444
CAAGGGT6300.0290.317477
ACTACCG12000.0290.083343
CTACTAG11600.0289.91384
ACTCGGG13800.0289.65586
Produced by FastQC (version 0.11.5)