FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005490745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70781
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.1426936607281615No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.1370424266399175No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.13280400107373447No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.13139119255167347No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.12997838402961245No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.12856557550755146No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.12291434141930745No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.12291434141930745No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.12150153289724644No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC860.12150153289724644No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.12008872437518543No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.11726310733106342No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.11726310733106342No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.11726310733106342No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.11585029880900241No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.11585029880900241No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.1144374902869414No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC810.1144374902869414No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.1130246817648804No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.1130246817648804No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.1130246817648804No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.1130246817648804No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC800.1130246817648804No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.1116118732428194No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.1116118732428194No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.1116118732428194No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.1116118732428194No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.1101990647207584No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.1101990647207584No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.10878625619869739No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.10878625619869739No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10737344767663638No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10737344767663638No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.10737344767663638No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.10596063915457538No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.10596063915457538No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.10596063915457538No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.10596063915457538No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.10596063915457538No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.10454783063251437No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.10454783063251437No Hit
TAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC740.10454783063251437No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10313502211045336No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10313502211045336No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10313502211045336No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10313502211045336No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC730.10313502211045336No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.10172221358839235No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.10172221358839235No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.10172221358839235No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.10172221358839235No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC720.10172221358839235No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.10030940506633136No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.10030940506633136No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.10030940506633136No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.10030940506633136No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.10030940506633136No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.10030940506633136No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC710.10030940506633136No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)