FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75388
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTAC4150.0295.000033
GCCCCTA1000.0295.02
GTCGCCT255.2568794E-10295.01
CTCCCTA700.0295.02
GGTCCTA750.0295.02
CGACCTA700.0295.02
CCTACGA255.2568794E-10295.05
GCGCCCT108.4493647E-4295.01
CGTTCCT206.146911E-8295.01
GACCCCT108.4493647E-4295.01
ACCTCGG108.4493647E-4295.04
GCACCCT255.2568794E-10295.01
CCCCCCT108.4493647E-4295.01
TAGGGGC108.4493647E-4295.07
GTTCCCT206.146911E-8295.01
CATACGG206.146911E-8295.05
GAGCCTA1000.0295.02
CTCGCCT108.4493647E-4295.01
ACGTCCT500.0295.01
TTGCCTA500.0295.02
Produced by FastQC (version 0.11.5)