FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72895
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC900.12346525824816516No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.11386240482886344No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.11386240482886344No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC810.11111873242334865No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC780.10700322381507647No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC750.10288771520680431No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC750.10288771520680431No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC740.10151587900404692No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC730.10014404280128952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCCT350.0295.000031
GAGTCCT350.0295.000031
GCGCCTA700.0295.000032
GCGCCCT350.0295.000031
GCACCCT350.0295.000031
TCTACCT350.0295.000031
ATCCCCT350.0295.000031
TCGTCCT700.0295.000031
TCCACCT350.0295.000031
TTGCCTA700.0295.000032
CACACCT350.0295.000031
AGGGCCT350.0295.000031
CCACCCT350.0295.000031
GTTGATT108.448789E-4295.0295
CCTTCCT303.6379788E-12295.01
AGTCCTA1000.0295.02
GATACCT108.448789E-4295.01
ATCTCCT206.145456E-8295.01
GTCGCCT206.145456E-8295.01
AGTCCCT157.212615E-6295.01
Produced by FastQC (version 0.11.5)