FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86469
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1530.17694202546577387No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1460.16884663867975808No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1380.15959476806716857No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1380.15959476806716857No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1370.1584382842405949No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1340.15496883276087384No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1340.15496883276087384No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1340.15496883276087384No Hit
TAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1320.15265586510772647No Hit
TATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1310.15149938128115278No Hit
GTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1310.15149938128115278No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1300.1503428974545791No Hit
ATCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1290.14918641362800542No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1280.14802992980143173No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1280.14802992980143173No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1270.14687344597485802No Hit
CCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1270.14687344597485802No Hit
CTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1240.14340399449513697No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1240.14340399449513697No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.14109102684198963No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1220.14109102684198963No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1210.1399345430154159No Hit
TTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1210.1399345430154159No Hit
AATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1210.1399345430154159No Hit
AAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1200.13877805918884226No Hit
ATTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1200.13877805918884226No Hit
CTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1200.13877805918884226No Hit
AAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1190.13762157536226857No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1190.13762157536226857No Hit
ATCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1190.13762157536226857No Hit
TCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1190.13762157536226857No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1190.13762157536226857No Hit
ATTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1190.13762157536226857No Hit
ACGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1180.13646509153569486No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1180.13646509153569486No Hit
ACTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.1353086077091212No Hit
TGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.1353086077091212No Hit
TCCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.1353086077091212No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.1353086077091212No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.1353086077091212No Hit
GCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.1353086077091212No Hit
CTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1170.1353086077091212No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1160.1341521238825475No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1160.1341521238825475No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1160.1341521238825475No Hit
CGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.1329956400559738No Hit
AATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.1329956400559738No Hit
TCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1150.1329956400559738No Hit
TTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.13068267240282644No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.13068267240282644No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.13068267240282644No Hit
CTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.13068267240282644No Hit
ATGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1130.13068267240282644No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.12952618857625275No Hit
TGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.12952618857625275No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.12952618857625275No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1120.12952618857625275No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.12836970474967907No Hit
TATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1110.12836970474967907No Hit
ACCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1100.12721322092310539No Hit
CAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.1260567370965317No Hit
TGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1090.1260567370965317No Hit
TTACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1080.12490025326995802No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.12374376944338435No Hit
TTTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1070.12374376944338435No Hit
CCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.12258728561681065No Hit
AAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1060.12258728561681065No Hit
GACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.12143080179023696No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1050.12143080179023696No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.12027431796366328No Hit
GCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.12027431796366328No Hit
TTGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.12027431796366328No Hit
CAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.12027431796366328No Hit
TGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.12027431796366328No Hit
CCACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1040.12027431796366328No Hit
CGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.1191178341370896No Hit
GGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.1191178341370896No Hit
TTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.1191178341370896No Hit
GCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1030.1191178341370896No Hit
CTCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.1179613503105159No Hit
TGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.1179613503105159No Hit
CCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.1179613503105159No Hit
CAAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.1179613503105159No Hit
TCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1020.1179613503105159No Hit
GCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.11680486648394223No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.11680486648394223No Hit
GTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.11680486648394223No Hit
CAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.11564838265736854No Hit
CGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1000.11564838265736854No Hit
TACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.11449189883079484No Hit
CATACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.11449189883079484No Hit
TGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.11449189883079484No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.11449189883079484No Hit
CGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC990.11449189883079484No Hit
AGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.11333541500422116No Hit
CGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.11333541500422116No Hit
TGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.11333541500422116No Hit
CCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.11333541500422116No Hit
CACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.11333541500422116No Hit
GAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC980.11333541500422116No Hit
GTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.11217893117764749No Hit
CTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC970.11217893117764749No Hit
TTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11102244735107379No Hit
AGTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11102244735107379No Hit
ATGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11102244735107379No Hit
GTTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11102244735107379No Hit
TTTGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC960.11102244735107379No Hit
GTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.10986596352450011No Hit
AACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.10986596352450011No Hit
CTGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.10986596352450011No Hit
TCGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.10986596352450011No Hit
ATCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.10986596352450011No Hit
GAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.10986596352450011No Hit
CCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.10986596352450011No Hit
AAGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC950.10986596352450011No Hit
TCCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.10870947969792644No Hit
GTCGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.10870947969792644No Hit
CTAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.10870947969792644No Hit
TCGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.10870947969792644No Hit
GGTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.10870947969792644No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.10870947969792644No Hit
GGGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.10870947969792644No Hit
ACTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.10870947969792644No Hit
TGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.10870947969792644No Hit
CACCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10755299587135274No Hit
TAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10755299587135274No Hit
ATACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10755299587135274No Hit
TCAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10755299587135274No Hit
TACGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC930.10755299587135274No Hit
AAGGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10639651204477904No Hit
GGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10639651204477904No Hit
CGCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.10639651204477904No Hit
ACACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.10524002821820537No Hit
ATAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.10524002821820537No Hit
AGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.10524002821820537No Hit
ACCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.10524002821820537No Hit
GGGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.10524002821820537No Hit
GCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.10524002821820537No Hit
TAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.10524002821820537No Hit
GATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.10408354439163169No Hit
TAAGCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.10408354439163169No Hit
CGCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.10408354439163169No Hit
TGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.10408354439163169No Hit
ATGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.10408354439163169No Hit
CGAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10292706056505799No Hit
AGCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10292706056505799No Hit
CCCCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10292706056505799No Hit
AAACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10292706056505799No Hit
AGATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.10292706056505799No Hit
CCGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.10177057673848432No Hit
TGTCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.10177057673848432No Hit
CTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC880.10177057673848432No Hit
GACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10061409291191063No Hit
AGACCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10061409291191063No Hit
CAGCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC870.10061409291191063No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTA950.0295.000032
CGTTCCT303.6379788E-12295.01
ATCCCCT303.6379788E-12295.01
GAGGCCT350.0295.01
TCCTCGG157.2172716E-6295.04
ACGCCTA1400.0295.02
TGCACCT255.275069E-10295.01
ACGCCCT157.2172716E-6295.01
CCTACGA157.2172716E-6295.05
ATCTCCT550.0295.01
AGCTCCT255.275069E-10295.01
TATACCT450.0295.01
TGACCTA1500.0295.02
CTCCCTA850.0295.02
AAATCCT500.0295.01
TTCGCCT303.6379788E-12295.01
TGCGCCT303.6379788E-12295.01
TTACCCT450.0295.01
AGGACCT255.275069E-10295.01
GTCGCCT303.6379788E-12295.01
Produced by FastQC (version 0.11.5)