FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71751
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT350.0295.000031
CGAACCT350.0295.000031
CGTACCT350.0295.000031
TTCGCCT350.0295.000031
TCGCCTA1000.0295.02
GCCCCTA750.0295.02
ACGCCCT157.212162E-6295.01
TTAGCCT450.0295.01
ACCGCCT255.2568794E-10295.01
ATAGCCT255.2568794E-10295.01
GACCCCT157.212162E-6295.01
GTTACCT255.2568794E-10295.01
AGGCCTA600.0295.02
GTAGCCT303.6379788E-12295.01
TACGCCT450.0295.01
GAGCCTA750.0295.02
CTCGCCT157.212162E-6295.01
GTATCCT255.2568794E-10295.01
CTACCCT255.2568794E-10295.01
ACGTCCT255.2568794E-10295.01
Produced by FastQC (version 0.11.5)