FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65441
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCT350.0295.000031
CCTACCT350.0295.000031
GTACCCT350.0295.000031
GAACCTA1400.0295.000032
TCTCCCT350.0295.000031
ATTCCTA1250.0295.000032
GGGCGGC350.0295.000039
CGCACCT350.0295.000031
AAGTCCT350.0295.000031
CGTTCCT108.446817E-4295.01
GTTGATT108.446817E-4295.0295
TTGTCCT206.140726E-8295.01
TGGTCCT108.446817E-4295.01
TGCACCT450.0295.01
TATACCT400.0295.01
CGCCCTA800.0295.02
GAAACCT255.2568794E-10295.01
AAATCCT206.140726E-8295.01
TTCGCCT206.140726E-8295.01
GATACCT255.2568794E-10295.01
Produced by FastQC (version 0.11.5)