FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78102
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC1020.13059844818314512No Hit
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC890.11395354792450897No Hit
ATAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC880.11267317098153697No Hit
AAGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC850.10883204015262092No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCC800.10243015543776088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTA950.0295.000032
TGATCCT206.148366E-8295.01
TCGCCTA750.0295.02
GCCCCTA700.0295.02
TCGCCCT108.4499555E-4295.01
GCCCCCT303.6379788E-12295.01
ATCCTAG108.4499555E-4295.03
AGTCCTA1800.0295.02
GTCGCCT206.148366E-8295.01
TGCCCCT303.6379788E-12295.01
CGAACCT255.275069E-10295.01
TCACCCT400.0295.01
CTTCCCT400.0295.01
CCTAGGG500.0295.05
AGCCCTA1050.0295.02
CCCTAGG157.214605E-6295.04
CCGGCCT255.275069E-10295.01
GATCCTA1000.0295.02
ATTACCT350.0295.01
CTCCCTA1050.0295.02
Produced by FastQC (version 0.11.5)