FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85398
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT303.6379788E-12295.01
CGAACCT350.0295.01
GCGACCT255.275069E-10295.01
GATCCTA1200.0295.02
GGTCCTA1600.0295.02
CGACCTA1100.0295.02
CGCGCCT400.0295.01
GCGCCCT350.0295.01
GACCCTA1050.0295.02
CGTTCCT450.0295.01
AACGGGA108.4513286E-4295.07
ACCTAGG255.275069E-10295.04
TCGCTTC157.2169496E-6295.0295
GTCACCT303.6379788E-12295.01
TACCTAG108.4513286E-4295.03
CTACCTA1400.0295.02
GGGCCTA500.0295.02
GAACCTA1550.0295.02
CGGTCCT400.0295.01
GGCGCCT350.0295.01
Produced by FastQC (version 0.11.5)