FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100112
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1450.14483778168451333No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1430.14284001917852007No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1340.13385008790155026No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1340.13385008790155026No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1320.13185232539555697No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1250.12486015662458046No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1250.12486015662458046No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1230.12286239411858717No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1220.12186351286559054No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1220.12186351286559054No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1220.12186351286559054No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1210.12086463161259389No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1210.12086463161259389No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1200.11986575035959725No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1190.1188668691066006No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1180.11786798785360397No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1180.11786798785360397No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1180.11786798785360397No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1150.11487134409461403No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1130.11287358158862074No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1110.11087581908262745No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1110.11087581908262745No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1100.10987693782963082No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1090.10887805657663417No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1090.10887805657663417No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1090.10887805657663417No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1090.10887805657663417No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1080.10787917532363754No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1080.10787917532363754No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1080.10787917532363754No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1070.10688029407064088No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1050.1048825315646476No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1050.1048825315646476No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1050.1048825315646476No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1050.1048825315646476No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1050.1048825315646476No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1050.1048825315646476No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1050.1048825315646476No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1040.10388365031165094No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1040.10388365031165094No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1040.10388365031165094No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1030.1028847690586543No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1020.10188588780565766No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1020.10188588780565766No Hit
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCC1020.10188588780565766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT305.456968E-12295.01
TCGCCCT305.456968E-12295.01
GGTCCTA1550.0295.02
CGCGCCT157.220673E-6295.01
GCGCCTA850.0295.02
GCGCCCT350.0295.01
GAGCCTA1050.0295.02
CTCGCCT350.0295.01
CGTACCT450.0295.01
GACGCCT500.0295.01
GGCGCCT255.275069E-10295.01
AGGTCCT600.0295.01
TTCTACG108.453509E-4294.999973
GATCCCT206.156915E-8294.999971
CGCTACG108.453509E-4294.999973
GGAACCT400.0294.999971
CCCCCCT650.0294.999971
GATGCCT108.453509E-4294.999971
CGCCCTA1450.0294.999972
TCCTACG27500.0293.390934
Produced by FastQC (version 0.11.5)