FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60097
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCTA850.0295.000032
AGCCTAC3300.0295.03
TGGTCCT255.2568794E-10295.01
ACGCCTA950.0295.02
CCTACCT350.0295.01
TAGTGCA255.2568794E-10295.0295
TGACCTA450.0295.02
TTTCCTA1300.0295.02
TTCGCCT255.2568794E-10295.01
AATTCCT450.0295.01
TGTCCCT255.2568794E-10295.01
GTTCCTA1000.0295.02
ACCACCT350.0295.01
CCACGGG255.2568794E-10295.05
CCGCCCT255.2568794E-10295.01
TAGCCCT450.0295.01
GAGCCTA650.0295.02
GTTTCCT255.2568794E-10295.01
GGTTCCT255.2568794E-10295.01
TAGTCCT450.0295.01
Produced by FastQC (version 0.11.5)