FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75243
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCG157.2139956E-6294.995363
CCTAGGA157.2139956E-6294.995365
GAGTCCT350.0294.995361
CCGGCCT157.2139956E-6294.995361
GATCCTA600.0294.995362
ATTACCT157.2139956E-6294.995361
CTCCCCT303.6379788E-12294.995361
AGGACCT303.6379788E-12294.995361
CCTACGA303.6379788E-12294.995365
CCTACCT303.6379788E-12294.995361
GCGCCCT303.6379788E-12294.995361
ACGGGGC157.2139956E-6294.995368
GGCCCCT350.0294.995361
AGAACCT157.2139956E-6294.995361
ATTCCCT157.2139956E-6294.995361
AAGCCTA850.0294.995362
GTAGCCT157.2139956E-6294.995361
ACCTAGG157.2139956E-6294.995364
AGACCCT350.0294.995361
TCGTCCT600.0294.995361
Produced by FastQC (version 0.11.5)