FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75243
Sequences flagged as poor quality0
Sequence length301
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA27123.6043219967305933No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA17552.3324428850524304No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA17532.32978483048257No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA14791.965631354411706No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA14001.8606381989022234No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA10951.4552848769985247No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA10741.4273753040149915No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA10571.4047818401711787No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA10521.398136703746528No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA10111.3436465850643915No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9541.2678920298233722No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA9311.2373244022699788No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9291.2346663477001183No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA7420.9861382454181784No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA7240.9622157542894355No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7120.9462674268702737No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA6780.9010804991826483No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA6700.8904482809032068No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA6640.8824741171936259No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6550.8705128716292545No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA6400.8505774623553022No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA6390.849248435070372No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA6090.8093776165224673No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5990.7960873436731656No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5760.7655197161197719No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5710.7588745796951212No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA5270.7003973791581941No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5190.6897651608787528No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5160.6857780790239624No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA4800.6379330967664766No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4700.6246428239171751No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA4660.6193267147774544No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA4650.6179976874925242No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4440.5900881145089909No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4420.5874300599391306No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4360.5794558962295496No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4240.5635075688103877No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA4150.5515463232460163No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA4080.5422431322515051No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3720.4943981499940193No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3590.4771207952899273No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3470.4611724678707654No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3470.4611724678707654No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3300.4385790040269527No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCA3260.433262894887232No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3080.4093404037584892No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA3080.4093404037584892No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2920.38807596719960663No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2920.38807596719960663No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2910.38674693991467646No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2900.3854179126297463No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2880.38275985805988594No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA2860.38010180349002565No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2830.3761147216352352No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA2770.3681405579256542No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2710.3601663942160732No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA2660.35352125779142246No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2560.3402309849421209No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2530.3362439030873304No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2510.33358584851747003No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2450.3256116848078891No Hit
GGACTACAAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT2380.316308493813378No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA2180.2897279481147748No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA2150.2857408662599843No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA2110.2804247571202637No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA2000.2658054569860319No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1970.2618183751312414No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1960.2604893478463113No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1920.25517323870659064No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1890.2511861568518002No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1880.24985712956687003No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1870.24852810228193986No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1870.24852810228193986No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1840.24454102042714937No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1840.24454102042714937No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1830.2432119931422192No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1780.2365668567175684No Hit
GGACTACAAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1750.23257977486277792No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1720.22859269300798743No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1700.22593463843812714No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1700.22593463843812714No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1690.22460561115319697No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1680.22327658386826682No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1550.20599922916417474No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1540.2046702018792446No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA1530.20334117459431442No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1490.19802506545459375No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1470.19536701088473346No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1440.19137992902994297No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA1370.18207673803543187No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA1330.17676062889571123No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1320.17543160161078108No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTGA1290.1714445197559906No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1280.17011549247106045No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1270.16878646518613027No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA1230.16347035604640964No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA1220.16214132876147946No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA1190.158154246906689No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1180.15682521962175885No Hit
GGACTACACGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1170.15549619233682865No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA1140.15150911048203822No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.15150911048203822No Hit
GGACTACCAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT1140.15150911048203822No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1120.14885105591217787No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1110.14752202862724773No Hit
GGACTACAAGGGTATCTATGACTCTTGTCAACGTATAAGCCTTCCTGTTG1090.1448639740573874No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1080.14353494677245723No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1080.14353494677245723No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1070.14220591948752706No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1070.14220591948752706No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTGA1060.14087689220259692No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA1040.1382188376327366No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA1030.13688981034780642No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA980.13024467392315564No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA980.13024467392315564No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA980.13024467392315564No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA970.1289156466382255No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA970.1289156466382255No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA930.12359953749850483No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA920.12227051021357468No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA920.12227051021357468No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA920.12227051021357468No Hit
GGACTACCAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT900.11961245564371435No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA890.1182834283587842No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA890.1182834283587842No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA890.1182834283587842No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA880.11695440107385405No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCA880.11695440107385405No Hit
GGACTACACGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT880.11695440107385405No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA870.1156253737889239No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGA860.11429634650399371No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCA850.11296731921906357No Hit
GGACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCA850.11296731921906357No Hit
GGACTACAAGGGTATCTAATCCCATTTGCTACCCTGGCTTTCGTTCCTCA830.11030926464920324No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCATGCTTTCGAGCCTCA830.11030926464920324No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA820.10898023736427309No Hit
GGACTACCCGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT820.10898023736427309No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCACGCTCTCGTGCATCA800.10632218279441277No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA800.10632218279441277No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA800.10632218279441277No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTGA800.10632218279441277No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.1049931555094826No Hit
GGACTACTAGGGTTTCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT780.10366412822455245No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCA780.10366412822455245No Hit
GGACTACTAGGGTATCTAATAAGGGTTTGGGGACCTGAAATAATCTCATT760.10100607365469212No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCC108.449331E-4295.0295
TCGGGTT1400.0295.08
ACCTGGG108.449331E-4295.06
CGGGGTA3250.0295.08
ACTCGGG4600.0295.06
TACTCGG4600.0295.05
ACATGGG108.449331E-4295.06
AGTTAGG108.449331E-4295.0295
GGACTAC69100.0294.786531
ACAAGGG17300.0293.29486
CAAGGGT17350.0292.449557
ACGGGTA4700.0291.86178
CTCGGGT4650.0291.827947
CACGGGT8950.0291.70397
CTACAAG17400.0291.60924
GGGGTAT8050.0291.33549
GACTACA33600.0291.04912
AGGGTAT20650.0290.714269
ACCAGGG9950.0290.552766
CTACCGG5950.0290.042024
Produced by FastQC (version 0.11.5)