FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58264
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCC630.10812851846766441No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCC600.10297954139777564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA750.0295.02
GCCCCTA700.0295.02
ATCCTCG108.4444473E-4295.03
TGCCCTA750.0295.02
TGCCCCT206.1349056E-8295.01
ACGCCTA650.0295.02
TAGACCT157.2052026E-6295.01
GATCCTA1050.0295.02
GGTCCTA700.0295.02
TTGTCCT108.4444473E-4295.01
GATCCCT108.4444473E-4295.01
GCGCCCT157.2052026E-6295.01
CGCGAGG108.4444473E-4295.09
TCCTAGG157.2052026E-6295.04
GCACCCT255.2568794E-10295.01
TACGCCT206.1349056E-8295.01
CGCTCCT303.6379788E-12295.01
TATTCCT350.0295.01
CGGGCCT157.2052026E-6295.01
TCCACCT350.0295.01
Produced by FastQC (version 0.11.5)