FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65860
Sequences flagged as poor quality0
Sequence length301
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC830.12602490130580019No Hit
TCCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC820.12450652900091103No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC790.11995141208624356No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC790.11995141208624356No Hit
AACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC770.11691466747646524No Hit
ACAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC770.11691466747646524No Hit
CACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC750.11387792286668692No Hit
TCGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC750.11387792286668692No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC740.11235955056179776No Hit
ATATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC740.11235955056179776No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC740.11235955056179776No Hit
CAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC730.1108411782569086No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC710.10780443364713028No Hit
TTGACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC710.10780443364713028No Hit
CTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC700.10628606134224113No Hit
TACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC700.10628606134224113No Hit
GTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC700.10628606134224113No Hit
AGGTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC690.10476768903735197No Hit
ACATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC680.10324931673246279No Hit
TATCCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC680.10324931673246279No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC670.10173094442757363No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC670.10173094442757363No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCC660.10021257212268447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT157.20947E-6295.01
ATCCTCG108.4469485E-4295.03
AATCCCT350.0295.01
GTCGCCT108.4469485E-4295.01
CCCCTAC3600.0295.03
TGCCCCT350.0295.01
CGAACCT157.20947E-6295.01
GCGACCT206.14109E-8295.01
CAACGGG108.4469485E-4295.06
ATGGACA255.2568794E-10295.0295
TTGTCCT400.0295.01
CAACCTA1700.0295.02
GGTCCCT108.4469485E-4295.01
CGCGCCT157.20947E-6295.01
CGACCCT206.14109E-8295.01
GCGCCTA600.0295.02
CCTACGA157.20947E-6295.05
ACTTACG108.4469485E-4295.04
CGTTCCT350.0295.01
GACCCCT157.20947E-6295.01
Produced by FastQC (version 0.11.5)