FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83255
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCTA1600.0295.000032
CGACCCT206.150731E-8295.000031
CCTACCG108.450954E-4295.000035
TGACGCC108.450954E-4295.00003295
AGCGCCT400.0295.000031
CTACCGG108.450954E-4295.000036
TCCTTTA108.450954E-4295.00003295
TGATCCT550.0295.01
TCGCCTA1300.0295.02
GCCCCCT157.216313E-6295.01
ACGCCTA1150.0295.02
ACGCCCT157.216313E-6295.01
ACCGCCT450.0295.01
TTGTCCT450.0295.01
CGTTCCT350.0295.01
TACGCCT350.0295.01
TGCGCCT255.275069E-10295.01
CTGCCTA1100.0295.02
TATTCCT700.0295.01
CGGGCCT350.0295.01
Produced by FastQC (version 0.11.5)