FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59267
Sequences flagged as poor quality0
Sequence length301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA19623.3104425734388445No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA19023.209205797492703No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA14542.453304537094842No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA13822.331820405959472No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA13582.2913256955810146No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA13322.2474564260043532No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA11922.011237282130022No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA11912.0095500025309194No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA10781.8188874078323518No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA10651.7969527730440211No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA9901.6704068031113437No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA9751.6450976091248082No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA9601.6197884151382724No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA9351.5776064251607134No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA8271.3953802284576577No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA8131.3717583140702245No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA7591.2806452157186967No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA7481.2620851401285706No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA7081.1945939561644758No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA7031.1861575581689643No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA6761.1406010089932002No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA6631.1186663742048695No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA6631.1186663742048695No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA6631.1186663742048695No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA6491.0950444598174363No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA6201.0461133514434677No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGCGCTTCA6191.0444260718443654No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA6051.0208041574569322No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA5300.8942581875242547No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA5160.8706362731368215No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA5120.863887154740412No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA5040.8503889179475931No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4470.7542139807987582No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4310.7272175072131203No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4100.6917846356319706No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4080.6884100764337658No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA4050.6833482376364587No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA4030.679973678438254No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3820.6445408068571042No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3820.6445408068571042No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3720.6276680108660806No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA3540.5972969780822379No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3500.5905478596858286No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA3430.578736902492112No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3290.5551149881046789No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA3220.5433040309109622No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA3160.533180353316348No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3050.514620277726222No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCA3010.5078711593298125No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA2700.45556549175763916No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA2610.4403799753657178No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA2560.431943577370206No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2430.4100089425818752No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2420.4083216629827729No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA2150.362765113807009No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA2140.36107783420790657No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2120.3577032750097019No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2100.3543287158114971No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA2070.34926687701419No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA2060.34757959741508765No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2030.34251775861778055No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2020.3408304790186782No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA2000.33745591982047346No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1850.31214672583393793No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1830.3087721666357332No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGA1810.3053976074375285No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1810.3053976074375285No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1730.2918993706447095No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1670.28177569305009537No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA1650.2784011338518906No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1640.2767138542527882No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1530.2581537786626622No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA1490.25140466026625274No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA1490.25140466026625274No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA1410.23790642347343377No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1370.2311573050770243No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1370.2311573050770243No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1320.2227209070815125No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA1280.215971788685103No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA1210.20416083149138645No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1210.20416083149138645No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1190.2007862722931817No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1140.19234987429766986No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA1130.1906625946985675No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1130.1906625946985675No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1120.18897531509946514No Hit
GGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1120.18897531509946514No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA1070.18053891710395328No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA1050.17716435790574855No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA1040.17547707830664622No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA1040.17547707830664622No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA1010.1704152395093391No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA990.16704068031113437No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA980.16535340071203197No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA980.16535340071203197No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA940.1586042823156225No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA910.15354244351831542No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA810.13666964752729174No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA780.13160780872998465No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA770.12992052913088228No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA770.12992052913088228No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA740.12485869033357518No Hit
GGACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA720.12148413113537045No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA710.11979685153626807No Hit
GGACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA690.11642229233806334No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA670.11304773313985861No Hit
GGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA670.11304773313985861No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA660.11136045354075624No Hit
GGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA660.11136045354075624No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCA650.10967317394165388No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCCTCA650.10967317394165388No Hit
GGACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCA640.1079858943425515No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA630.10629861474344914No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTTA610.1029240555452444No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA600.10123677594614203No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGA600.10123677594614203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA3250.0295.000038
TACTAGG7700.0295.000035
ACTCGGG5900.0295.06
CGGGGTA1700.0295.08
AATTCGC255.2568794E-10295.0295
ACCCGGG4750.0295.06
CTTAGCC108.444813E-4295.0295
TACACGG7950.0295.05
GGTTAGC157.2058265E-6295.0295
CAGGGTA3750.0295.08
ACGGGTT4750.0295.08
TTTAGTC303.6379788E-12295.0295
TTTAGAC157.2058265E-6295.0295
AGTTGGC206.135815E-8295.0295
CTCGGGT5900.0295.07
TACTCGG5900.0295.05
CTACCCG4750.0295.04
CTACTCG5850.0295.04
TCGGGTT2600.0295.08
GGACTAC56000.0294.73661
Produced by FastQC (version 0.11.5)