FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65921
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT350.0295.000031
GCCCCTA700.0295.000032
ACGCCTA700.0295.000032
GATCCTA850.0295.000032
GTCGCCT108.446963E-4295.01
CCCCTCG108.446963E-4295.03
CGTGCCT108.446963E-4295.01
CGCGCCT108.446963E-4295.01
CGTTCCT450.0295.01
AGAACCT255.2568794E-10295.01
CCGTCCT206.14109E-8295.01
TTCCCTA950.0295.02
CGGGGCA108.446963E-4295.09
ACCTAGG255.2568794E-10295.04
TACGCCT255.2568794E-10295.01
CCCCCTA1150.0295.02
GAGCCTA800.0295.02
CTCGCCT108.446963E-4295.01
CGAGCCT206.14109E-8295.01
TTACGGG108.446963E-4295.06
Produced by FastQC (version 0.11.5)