FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57203
Sequences flagged as poor quality0
Sequence length301
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT303.6379788E-12295.01
GCCCCTA500.0295.02
GTCGCCT157.204524E-6295.01
CCCCTAC3100.0295.03
ACGCCTA750.0295.02
TAGACCT206.133996E-8295.01
GATCCTA900.0295.02
ATTACCT303.6379788E-12295.01
GGTCCTA700.0295.02
CCTACGA157.204524E-6295.05
GCGCCCT350.0295.01
ACCCTTT157.204524E-6295.0295
TAGCCTA500.0295.02
GTAGCCT108.44405E-4295.01
ACCTAGG255.2568794E-10295.04
CTCGGAG108.44405E-4295.06
CTACGAG157.204524E-6295.06
CCCCCTA650.0295.02
TACCTAG157.204524E-6295.03
GGGCCTA650.0295.02
Produced by FastQC (version 0.11.5)