FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75229
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC1010.13425673609911074No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC940.12495181379521196No Hit
TTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.1222932645655266No Hit
TTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC920.1222932645655266No Hit
GTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC910.12096398995068393No Hit
TCTACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC900.11963471533584123No Hit
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC890.11830544072099855No Hit
CCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC850.11298834226162782No Hit
CATTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.11165906764678515No Hit
AACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC840.11165906764678515No Hit
CTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC830.11032979303194246No Hit
CCATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC820.10900051841709979No Hit
CTCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.10501269457257176No Hit
ACCACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC790.10501269457257176No Hit
TTCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC780.10368341995772906No Hit
TCTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.1023541453428864No Hit
ATCTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC770.1023541453428864No Hit
TCAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.1010248707280437No Hit
TACACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC760.1010248707280437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACCT850.0295.000031
ATCCCCT350.0295.01
ACAGCCT350.0295.01
ATCCTCG108.449331E-4295.03
TGGTCCT350.0295.01
TCCTCGG255.2568794E-10295.04
AACGCCT255.2568794E-10295.01
ACGCCTA1150.0295.02
GGGTCCT206.146729E-8295.01
AATCCCT350.0295.01
GTCACCT450.0295.01
AGCTCCT255.2568794E-10295.01
CACACCT400.0295.01
TAGTGCA255.2568794E-10295.0295
TGACCTA1300.0295.02
CTCCCTA1150.0295.02
GAAACCT400.0295.01
TTTCCTA1400.0295.02
TTCGCCT500.0295.01
TCCTAGG255.2568794E-10295.04
Produced by FastQC (version 0.11.5)