FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66670
Sequences flagged as poor quality0
Sequence length301
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA24763.7138143092845355No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA18332.7493625318734063No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA17742.6608669566521677No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA14372.1553922303884807No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA13201.9799010049497527No Hit
GGACTACAAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA12851.9274036298185089No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA12771.9154042297885105No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA12511.8764061796910154No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9941.4909254537273136No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA9761.463926803659817No Hit
GGACTACTAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA9751.4624268786560672No Hit
GGACTACCAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA9711.456427178641068No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9681.4519274036298184No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9461.4189290535473227No Hit
GGACTACACGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA9311.3964301784910755No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8351.2524373781310933No Hit
GGACTACAGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA8291.2434378281085945No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8161.223938803059847No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7661.1489425528723565No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA7491.1234438278086096No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7331.0994450277486125No Hit
GGACTACTAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA7241.0859457027148642No Hit
GGACTACACGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA6981.046947652617369No Hit
GGACTACCAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA6921.0379481025948702No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6881.031948402579871No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA6300.9449527523623819No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6290.9434528273586321No Hit
GGACTACAGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA6070.9104544772761363No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5970.895455227238638No Hit
GGACTACTCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5810.871456427178641No Hit
GGACTACCCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5660.8489575521223938No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5190.7784610769461526No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5180.7769611519424029No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5130.7694615269236539No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5100.7649617519124045No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA4970.7454627268636568No Hit
GGACTACTGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA4950.7424628768561572No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4800.7199640017999099No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4780.7169641517924104No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA4590.6884655767211639No Hit
GGACTACCGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA4500.6749662516874156No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4160.623968801559922No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4030.6044697765111745No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3960.5939703014849257No Hit
GGACTACTCGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA3930.5894705264736763No Hit
GGACTACCCGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA3880.5819709014549272No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3840.575971201439928No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3760.5639718014099295No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3650.5474726263686815No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3560.5339733013349333No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3550.5324733763311834No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3490.5234738263086846No Hit
GGACTACTGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA3250.487475626218689No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3140.4709764511774411No Hit
GGACTACCGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA3130.46947652617369134No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2990.448477576121194No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA2950.4424778761061947No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2940.44097795110244487No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2920.4379781010949452No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2870.4304784760761962No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2750.41247937603119844No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA2730.4094795260236988No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA2730.4094795260236988No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2700.4049797510124494No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA2490.3734813259337033No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2440.36598170091495424No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2410.3614819259037048No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA2300.3449827508624569No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2290.34348282585870704No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA2230.3344832758362082No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2140.3209839508024599No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA2110.31648417579121046No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2040.30598470076496176No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2010.30148492575371233No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1910.2864856757162142No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA1910.2864856757162142No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1840.2759862006899655No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1800.26998650067496627No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1800.26998650067496627No Hit
GGACTACAAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1770.2654867256637168No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1730.25948702564871756No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA1720.25798710064496777No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA1610.2414879256037198No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1600.23998800059997No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1600.23998800059997No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1590.2384880755962202No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1550.23248837558122096No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1540.2309884505774711No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA1520.2279886005699715No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1490.22348882555872207No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1440.21598920053997303No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1410.21148942552872357No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1360.20398980050997448No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1300.19499025048747562No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1260.18899055047247637No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1220.18299085045747712No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA1220.18299085045747712No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1190.1784910754462277No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.17099145042747863No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1140.17099145042747863No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA1130.1694915254237288No Hit
GGACTACAAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1100.16499175041247938No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1060.15899205039748013No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1050.1574921253937303No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA990.14849257537123142No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA970.1454927253637318No Hit
GGACTACACGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA940.14099295035248238No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA930.13949302534873256No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA930.13949302534873256No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA900.13499325033748313No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA900.13499325033748313No Hit
GGACTACCAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA890.13349332533373331No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA880.1319934003299835No Hit
GGACTACTAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA870.13049347532623368No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA870.13049347532623368No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA850.12749362531873407No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA850.12749362531873407No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA840.12599370031498425No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA830.12449377531123444No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA820.12299385030748462No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA820.12299385030748462No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA770.11549422528873556No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA770.11549422528873556No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA750.11249437528123593No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA730.1094945252737363No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA730.1094945252737363No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA730.1094945252737363No Hit
GGACTACACGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA710.10649467526623668No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA700.10499475026248686No Hit
GGACTACAGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA700.10499475026248686No Hit
GGACTACCAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA670.10049497525123745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAC206.1187166E-8295.220731
GGACTAT108.4282644E-4295.220731
ACTCGGG4800.0294.999276
GTTTGCC350.0294.99927295
CGGGGTA2000.0294.999278
AGGGTTC157.2099374E-6294.999279
ACGGGTA4400.0294.999278
GGTAGCC157.2099374E-6294.99927295
CTCGGGT4800.0294.999277
TACTCGG4800.0294.999275
GTTATCC255.2568794E-10294.99927295
CTACAAG15350.0294.999244
AATTCGC108.4472436E-4294.99924295
TACACGG8600.0294.999245
TACAAGG15350.0294.999245
CCACAAG108.4472436E-4294.999244
AATTGGC108.4472436E-4294.99924295
GGACTAC63550.0294.988431
ACAAGGG15450.0294.044566
CGGGTAT9350.0293.421729
Produced by FastQC (version 0.11.5)