FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005490826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70557
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA21333.0230877163144694No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA17832.52703487960089No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA13811.957282764289865No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA13651.9346060631829582No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA13291.883583485692419No Hit
GGACTACAAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA13131.8609067845855125No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA12701.7999631503607012No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA12461.7659480987003415No Hit
GGACTACACGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA12341.7489405728701617No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA12271.7390195161358903No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA12241.7347676346783452No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9691.3733577107870232No Hit
GGACTACAAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA9311.3195005456581204No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA9291.316665958019757No Hit
GGACTACCAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA9171.2996584321895772No Hit
GGACTACAGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA8671.2287937412304943No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8671.2287937412304943No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8591.2174553906770413No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8261.1706846946440466No Hit
GGACTACTAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA8201.1621809317289566No Hit
GGACTACCCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA8121.1508425811755036No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA8021.1366696429836871No Hit
GGACTACACGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA7591.0757260087588758No Hit
GGACTACTCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA7441.054466601471151No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA7361.0431282509176976No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA7181.017616962172428No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA6720.9524214464900718No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA6570.9311620392023471No Hit
GGACTACAAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA6370.9028161628187139No Hit
GGACTACCGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA6080.861714642062446No Hit
GGACTACCAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5750.8149439460294514No Hit
GGACTACAGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5660.8021883016568164No Hit
GGACTACCCGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5550.7865980696458182No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5520.7823461881882734No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA5370.7610867809005485No Hit
GGACTACTGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA5180.7341581983360971No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA5090.7214025539634622No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4790.6788837393880125No Hit
GGACTACTCGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA4780.6774664455688308No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4750.6732145641112859No Hit
GGACTACACGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA4750.6732145641112859No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4720.6689626826537409No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4660.660458919738651No Hit
GGACTACCGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA4560.6462859815468345No Hit
GGACTACTAGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA4530.6420341000892895No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA4310.6108536360672931No Hit
GGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3810.5399889451082104No Hit
GGACTACCAGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3790.5371543574698471No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3790.5371543574698471No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3760.5329024760123021No Hit
GGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3720.5272333007355755No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3670.5201468316396671No Hit
GGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3570.5059738934478507No Hit
GGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3510.49747013053276073No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3370.4776280170642176No Hit
GGACTACTGGGGTTTCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCA3360.4762107232450359No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3280.46487237269158266No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3220.4563686097764928No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3210.45495131595731114No Hit
GGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA3170.44928214068058453No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3160.4478648468614029No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA3070.43510920248876794No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2750.389755800274955No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2700.3826693311790467No Hit
GGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA2700.3826693311790467No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2630.37274827444477515No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA2530.35857533625295857No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2460.34865427951868705No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA2410.34156781042277873No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2370.3358986351460521No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA2350.3330640475076888No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA2290.3245602845925989No Hit
GGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA2290.3245602845925989No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2260.32030840313505393No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2230.316056521677509No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA2190.31038734640078236No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2170.30755275876241905No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2030.2877106452938759No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCCTCA1930.2735377071020593No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1860.26361665036778775No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA1840.2607820627294244No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1820.25794747509106114No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1780.25227829981433453No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA1700.24093994926088128No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1630.23101889252660968No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1600.22676701106906474No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1530.21684595433479315No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1510.21401136669642984No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1510.21401136669642984No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1500.21259407287724819No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1490.21117677905806653No Hit
GGACTACAAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1480.20975948523888488No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1470.2083421914197032No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACCTTCGAGCTTCA1460.20692489760052155No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1430.2026730161429766No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1420.20125572232379493No Hit
GGACTACACGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1400.19842113468543165No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1340.1899173717703417No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1300.1842481964936151No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1220.17290984594016187No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1210.17149255212098022No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1200.17007525830179854No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1200.17007525830179854No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1200.17007525830179854No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1170.16582337684425358No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1170.16582337684425358No Hit
GGACTACTAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1130.16015420156752697No Hit
GGACTACAAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1130.16015420156752697No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1120.1587369077483453No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1090.15448502629080035No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1070.15165043865243705No Hit
GGACTACAGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA1070.15165043865243705No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1060.15023314483325537No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1050.1488158510140737No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1040.14739855719489206No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA1040.14739855719489206No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1030.1459812633757104No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA1020.14456396955652878No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA1010.14314667573734713No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA1010.14314667573734713No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA990.1403120880989838No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA990.1403120880989838No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA970.1374775004606205No Hit
GGACTACCCGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA960.13606020664143884No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA960.13606020664143884No Hit
GGACTACCAGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA960.13606020664143884No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA950.13464291282225718No Hit
GGACTACTCGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA940.13322561900307553No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA910.12897373754553057No Hit
GGACTACACGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA910.12897373754553057No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA880.12472185608798561No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA880.12472185608798561No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA870.12330456226880394No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA860.12188726844962229No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA840.11905268081125897No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCA830.11763538699207732No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA810.11480079935371401No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA800.11338350553453237No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA790.11196621171535072No Hit
GGACTACCGGGGTATCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA780.11054891789616907No Hit
GGACTACCAGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA760.10771433025780575No Hit
GGACTACCCGGGTTTCTAATCCCGTTTGCTACCCTGGCTTTCGCATCTCA760.10771433025780575No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA750.10629703643862409No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCACACTTTCGAGCATCA750.10629703643862409No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCA730.10346244880026077No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTGA730.10346244880026077No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGA720.10204515498107912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)