FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62428
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1200.19222143909784073No Hit
TCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1090.174601140513872No Hit
ACATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1050.1681937592106106No Hit
CCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1050.1681937592106106No Hit
AAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1040.16659191388479527No Hit
TTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1020.1633882232331646No Hit
ATCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1010.1617863779073493No Hit
CACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.16018453258153392No Hit
TTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.16018453258153392No Hit
TTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC1000.16018453258153392No Hit
CTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC990.15858268725571859No Hit
AACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC980.15698084192990325No Hit
TTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC980.15698084192990325No Hit
TTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC980.15698084192990325No Hit
TTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC970.1553789966040879No Hit
ACGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.1505734606266419No Hit
ACACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC940.1505734606266419No Hit
GTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC930.14897161530082653No Hit
CAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.14736976997501122No Hit
TACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC920.14736976997501122No Hit
CTAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.14416607932338052No Hit
CTCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.14416607932338052No Hit
TTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC900.14416607932338052No Hit
ATCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.1425642339975652No Hit
AGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.1425642339975652No Hit
CACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.1425642339975652No Hit
ATTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.1425642339975652No Hit
CCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC890.1425642339975652No Hit
ATACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.14096238867174984No Hit
ACTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.14096238867174984No Hit
ATAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.14096238867174984No Hit
TCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC880.14096238867174984No Hit
GTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.13936054334593453No Hit
AAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.13936054334593453No Hit
TCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC870.13936054334593453No Hit
GTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.13775869802011917No Hit
CACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.13775869802011917No Hit
CTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC860.13775869802011917No Hit
TAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.13615685269430383No Hit
AGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.13615685269430383No Hit
ATTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.13615685269430383No Hit
ATATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC850.13615685269430383No Hit
TAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.1345550073684885No Hit
TTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC840.1345550073684885No Hit
TTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.13295316204267316No Hit
AATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.13295316204267316No Hit
TTAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.13295316204267316No Hit
TTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.13295316204267316No Hit
TATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC830.13295316204267316No Hit
CTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.13135131671685782No Hit
CCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.13135131671685782No Hit
ATGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.13135131671685782No Hit
ACTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC820.13135131671685782No Hit
TCCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.12974947139104248No Hit
GTATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC810.12974947139104248No Hit
TAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12814762606522714No Hit
GAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12814762606522714No Hit
ATGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12814762606522714No Hit
GTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12814762606522714No Hit
ATAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC800.12814762606522714No Hit
GTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.1265457807394118No Hit
TCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.1265457807394118No Hit
CCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.1265457807394118No Hit
CCCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC790.1265457807394118No Hit
GTTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12494393541359647No Hit
GGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12494393541359647No Hit
CGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12494393541359647No Hit
GGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12494393541359647No Hit
GTCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12494393541359647No Hit
TAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12494393541359647No Hit
CTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC780.12494393541359647No Hit
AGATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC770.12334209008778112No Hit
GCACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1217402447619658No Hit
TTTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1217402447619658No Hit
CTTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1217402447619658No Hit
CATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1217402447619658No Hit
GGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC760.1217402447619658No Hit
CGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.12013839943615044No Hit
CAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.12013839943615044No Hit
GGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.12013839943615044No Hit
TGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC750.12013839943615044No Hit
ACAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.1185365541103351No Hit
GAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.1185365541103351No Hit
CAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.1185365541103351No Hit
CATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.1185365541103351No Hit
ACAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC740.1185365541103351No Hit
CAATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11693470878451978No Hit
TCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11693470878451978No Hit
ATCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11693470878451978No Hit
GCATCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11693470878451978No Hit
ACCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11693470878451978No Hit
GGCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11693470878451978No Hit
TGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11693470878451978No Hit
GAAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC730.11693470878451978No Hit
AATTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11533286345870443No Hit
TGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11533286345870443No Hit
GGGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11533286345870443No Hit
CTACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11533286345870443No Hit
AGGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11533286345870443No Hit
TACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11533286345870443No Hit
TCAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC720.11533286345870443No Hit
ACTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11373101813288908No Hit
ACCACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11373101813288908No Hit
GGACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11373101813288908No Hit
CGCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11373101813288908No Hit
TCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11373101813288908No Hit
TACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC710.11373101813288908No Hit
TCGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11212917280707375No Hit
AACCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11212917280707375No Hit
CTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC700.11212917280707375No Hit
GGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1105273274812584No Hit
TTGGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1105273274812584No Hit
CAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1105273274812584No Hit
GATACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1105273274812584No Hit
TCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1105273274812584No Hit
CTGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1105273274812584No Hit
TGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC690.1105273274812584No Hit
CGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10892548215544307No Hit
TAGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10892548215544307No Hit
CTGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10892548215544307No Hit
AAGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10892548215544307No Hit
AGTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10892548215544307No Hit
AGGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10892548215544307No Hit
CCGTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10892548215544307No Hit
TTGCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC680.10892548215544307No Hit
TACGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10732363682962774No Hit
TCCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10732363682962774No Hit
ACGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10732363682962774No Hit
AAAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10732363682962774No Hit
CCGACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC670.10732363682962774No Hit
TGTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10572179150381239No Hit
GGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10572179150381239No Hit
GACACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10572179150381239No Hit
ATTCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10572179150381239No Hit
TAAGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC660.10572179150381239No Hit
GTCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10411994617799707No Hit
AAACCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10411994617799707No Hit
ATCGCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10411994617799707No Hit
CCTACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC650.10411994617799707No Hit
AACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10251810085218171No Hit
GGCCCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC640.10251810085218171No Hit
GCAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10091625552636636No Hit
TGAACCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10091625552636636No Hit
GCTTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10091625552636636No Hit
CGCTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCC630.10091625552636636No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCT108.445888E-4295.01
GCCCCTA1000.0295.02
GCCCCCT206.1383616E-8295.01
AGTCCTA1100.0295.02
GTCGCCT206.1383616E-8295.01
TGCCCCT255.2568794E-10295.01
CGAACCT350.0295.01
CTTCCCT350.0295.01
GAGTCCT206.1383616E-8295.01
CCGGCCT108.445888E-4295.01
GAGTACT108.445888E-4295.0295
GATCCTA900.0295.02
GGTCCTA850.0295.02
CGCGCCT206.1383616E-8295.01
GCGCCTA650.0295.02
CTCCACG108.445888E-4295.03
TACGGGG700.0295.07
TACGGCG400.0295.07
TAGCCTA1000.0295.02
CGTTCCT206.1383616E-8295.01
Produced by FastQC (version 0.11.5)