FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61318
Sequences flagged as poor quality0
Sequence length301
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC680.11089728953977623No Hit
ATAACCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCC640.10437391956684824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA950.0295.000032
CTCCCTA950.0295.000032
GGTCCTA950.0295.000032
GTGTCGG950.0295.00003295
TGATCCT450.0295.01
GCCCCCT108.4455253E-4295.01
GTCGCCT350.0295.01
CGAACCT400.0295.01
GAGTCCT108.4455253E-4295.01
GTCCTAC3600.0295.03
ATTACCT255.2568794E-10295.01
ATAGCCT108.4455253E-4295.01
GCGCCTA900.0295.02
CCTACGA108.4455253E-4295.05
GCGCCCT108.4455253E-4295.01
CGTTCCT255.2568794E-10295.01
TAGCCCT108.4455253E-4295.01
GACCCCT206.137634E-8295.01
AGAACCT500.0295.01
GCACCTA1000.0295.02
Produced by FastQC (version 0.11.5)