FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65236
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC750.1149671960267337No Hit
TTAACCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC680.1042369243975719No Hit
TTTTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10117113250352565No Hit
CTATCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTC660.10117113250352565No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTA1250.0295.000032
TCGCCTA700.0295.02
GTTGATT108.446757E-4295.0295
ACGCCTA800.0295.02
GTACAGG157.2091443E-6295.0295
CTCCCTA800.0295.02
TTGTCCT500.0295.01
ATAGCCT108.446757E-4295.01
TCCCTAC3000.0295.03
AAGACCT206.1405444E-8295.01
CACGCCT255.2568794E-10295.01
CGCGCCT206.1405444E-8295.01
GCGCCTA550.0295.02
CCTCGGA108.446757E-4295.05
ACGGGGC157.2091443E-6295.08
GACCCCT206.1405444E-8295.01
ACCTCGG206.1405444E-8295.04
CACCTCG108.446757E-4295.03
GTTACCT400.0295.01
ATTCCCT206.1405444E-8295.01
Produced by FastQC (version 0.11.5)