FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91018
Sequences flagged as poor quality0
Sequence length301
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA950.0295.02
GCCCCTA1050.0295.02
GCCCCCT303.6379788E-12295.01
GTCGCCT206.153823E-8295.01
CCCCTAC5500.0295.03
AGATCCT255.275069E-10295.01
ACGCCTA1250.0295.02
GTCGATA108.452246E-4295.0295
CAGCCTA900.0295.02
GAGTCCT303.6379788E-12295.01
ATTACCT550.0295.01
TTGTCCT350.0295.01
GTCTCCT157.2185176E-6295.01
GGTCCCT255.275069E-10295.01
AAGACCT255.275069E-10295.01
CACGCCT450.0295.01
CGCGCCT206.153823E-8295.01
GGATCCT350.0295.01
CCTACGT108.452246E-4295.05
CCTAAGG157.2185176E-6295.05
Produced by FastQC (version 0.11.5)