FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005490857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005490857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76103
Sequences flagged as poor quality0
Sequence length301
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAC2800.0295.000033
GATTCCT350.0295.000031
TGATCCT303.6379788E-12295.01
ACGCCCT255.2568794E-10295.01
ACACCTA1300.0295.02
CGCGCCT255.2568794E-10295.01
GGATCCT303.6379788E-12295.01
GCGCCTA500.0295.02
GCGCCCT157.213879E-6295.01
CGCCTCG206.147275E-8295.03
GTAGCCT206.147275E-8295.01
TCGGAAA255.2568794E-10295.0295
TACGCCT157.213879E-6295.01
CTACGAG108.4495306E-4295.06
CGGGCCT206.147275E-8295.01
GTTCCCT157.213879E-6295.01
GGGCCTA850.0295.02
CGAGCCT108.4495306E-4295.01
CCGACCT255.2568794E-10295.01
GACGCCT157.213879E-6295.01
Produced by FastQC (version 0.11.5)